13.3 Discussion We have demonstrated that microfluidics provides a flexibility in the study of cellular mechanics at a relatively high throughput compared to existing methods. The sickle cell microvascular model has been designed to achieve specific measurements under physiological conditions mimicking cellular environment in vivo, such as flow, oxygen level and temperature. Relationships between in vitro microfluidics measurements and cellular disease states have been established. This model can be further improved for more comprehensive study of single cells and cell-cell interactions. For instance, in the model of vascular occlusion in sickle cell disease, we demonstrated the cellular velocity and capillary obstructions in response to oxygen changes. 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